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EP300
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  • EP300
PROTEIN STRUCTURE
ANTIBODIES
AND
VALIDATION
Protein structures
GENERAL INFORMATIONi

General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.

Gene namei

Official gene symbol, which is typically a short form of the gene name, according to HGNC.

EP300
Synonyms KAT3B, p300
Gene descriptioni

Full gene name according to HGNC.

E1A binding protein p300
Protein classi

Assigned HPA protein class(es) for the encoded protein(s).

Read more
Cancer-related genes
Disease related genes
Enzymes
Human disease related genes
Metabolic proteins
Potential drug targets
Predicted locationi

All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions.

  • Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached.

  • Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s).

The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location.

Read more
Intracellular
Protein evidence Evidence at protein level (all genes)
GENE INFORMATIONi

Gene information from Ensembl and Entrez, as well as links to available gene identifiers are displayed here. Information was retrieved from Ensembl if not indicated otherwise.

Chromosome 22
Cytoband q13.2
Chromosome location (bp) 41092592 - 41180077
Number of transcriptsi

Number of protein-coding transcripts from the gene as defined by Ensembl.

3
Ensembl ENSG00000100393 (version 103.38)
Entrez gene 2033
HGNC HGNC:3373
UniProt Q09472 (UniProt - Evidence at protein level)
neXtProt NX_Q09472
Antibodypedia EP300 antibodies


PROTEIN STRUCTUREi

The Structure section provides predicted structures from the Alphafold protein structure database and available experimental structures from Protein Data Bank (PDB).

In the Structure drop-down menu all experimental structures from PDB are available for selection and display. The structures are displayed using the NGL Viewer and can be zoomed-in and rotated either manually or by checking the Autorotate box. The Color scheme can be selected to show the residue index, chain name or confidence score (as B-factors and pLDDT score for experimental and predicted structures, respectively). The positions for available antigen sequences in the structure are shown if Antigens is turned to ON, and the Variants slider can be used to show the positions of clinical and population variants.https://github.com/nglviewer/ngl

Read more

Predicted


Description: Structure prediction from Alphafold project. Structure version 2

# Chains: 1      # Clinical variants: 10      # Population variants: 1327

Antigens:

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On

Variants:

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Clinical
Population

Color scheme:

Confidence
Residue index
Chain name

Autorotate:

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On



PROTEIN BROWSERi

The protein browser displays the antigen location on the target protein(s) and the features of the target protein. The tabs at the top of the protein view section can be used to switch between the different splice variants to which an antigen has been mapped.

At the top of the view, the position of the antigen (identified by the corresponding HPA identifier) is shown as a green bar. A yellow triangle on the bar indicates a <100% sequence identity to the protein target.

Below the antigens, the maximum percent sequence identity of the protein to all other proteins from other human genes is displayed, using a sliding window of 10 aa residues (HsID 10) or 50 aa residues (HsID 50). The region with the lowest possible identity is always selected for antigen design, with a maximum identity of 60% allowed for designing a single-target antigen (read more).

The curve in blue displays the predicted antigenicity i.e. the tendency for different regions of the protein to generate an immune response, with peak regions being predicted to be more antigenic.The curve shows average values based on a sliding window approach using an in-house propensity scale. (read more).

If a signal peptide is predicted by a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius (turquoise) and/or transmembrane regions (orange) are predicted by MDM, these are displayed.

Low complexity regions are shown in yellow and InterPro regions in green. Common (purple) and unique (grey) regions between different splice variants of the gene are also displayed (read more), and at the bottom of the protein view is the protein scale.
EP300-201
EP300-203
EP300-212


PROTEIN INFORMATIONi

The protein information section displays alternative protein-coding transcripts (splice variants) encoded by this gene according to the Ensembl database.

The ENSP identifier links to the Ensembl website protein summary, while the ENST identifier links to the Ensembl website transcript summary for the selected splice variant. The data in the UniProt column can be expanded to show links to all matching UniProt identifiers for this protein.

The protein classes assigned to this protein are shown if expanding the data in the protein class column. Parent protein classes are in bold font and subclasses are listed under the parent class.

The Gene Ontology terms assigned to this protein are listed if expanding the Gene ontology column. The length of the protein (amino acid residues according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius) and the number of predicted transmembrane region(s) (according to MDM) are also reported.
Splice variant UniProt Protein class Gene ontology Length & mass Signal peptide
(predicted)
Transmembrane regions
(predicted)
EP300-201
ENSP00000263253
ENST00000263253
Q09472 [Direct mapping]
Histone acetyltransferase p300
Show all
Enzymes
   ENZYME proteins
   Transferases
Metabolic proteins
Predicted intracellular proteins
   Intracellular proteins predicted by MDM and MDSEC
Cancer-related genes
   Mutated cancer genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Other Mutations
   COSMIC Nonsense Mutations
   COSMIC Missense Mutations
   COSMIC Frameshift Mutations
   COSMIC Translocations
Disease related genes
Potential drug targets
Human disease related genes
   Cancers
   Cancers of haematopoietic and lymphoid tissues
   Congenital malformations
   Other congenital malformations
Mapped to neXtProt
   neXtProt - Evidence at protein level
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000122 [negative regulation of transcription by RNA polymerase II]
GO:0000123 [histone acetyltransferase complex]
GO:0000977 [RNA polymerase II transcription regulatory region sequence-specific DNA binding]
GO:0000978 [RNA polymerase II cis-regulatory region sequence-specific DNA binding]
GO:0001085 [RNA polymerase II transcription factor binding]
GO:0001102 [RNA polymerase II activating transcription factor binding]
GO:0001666 [response to hypoxia]
GO:0001756 [somitogenesis]
GO:0001966 [thigmotaxis]
GO:0002039 [p53 binding]
GO:0002209 [behavioral defense response]
GO:0002223 [stimulatory C-type lectin receptor signaling pathway]
GO:0003677 [DNA binding]
GO:0003682 [chromatin binding]
GO:0003684 [damaged DNA binding]
GO:0003712 [transcription coregulator activity]
GO:0003713 [transcription coactivator activity]
GO:0004402 [histone acetyltransferase activity]
GO:0004468 [lysine N-acetyltransferase activity, acting on acetyl phosphate as donor]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005667 [transcription regulator complex]
GO:0005694 [chromosome]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0006110 [regulation of glycolytic process]
GO:0006283 [transcription-coupled nucleotide-excision repair]
GO:0006355 [regulation of transcription, DNA-templated]
GO:0006357 [regulation of transcription by RNA polymerase II]
GO:0006473 [protein acetylation]
GO:0006475 [internal protein amino acid acetylation]
GO:0006915 [apoptotic process]
GO:0006977 [DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest]
GO:0006990 [positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response]
GO:0007049 [cell cycle]
GO:0007219 [Notch signaling pathway]
GO:0007221 [positive regulation of transcription of Notch receptor target]
GO:0007399 [nervous system development]
GO:0007507 [heart development]
GO:0007519 [skeletal muscle tissue development]
GO:0007611 [learning or memory]
GO:0007623 [circadian rhythm]
GO:0008013 [beta-catenin binding]
GO:0008022 [protein C-terminus binding]
GO:0008134 [transcription factor binding]
GO:0008270 [zinc ion binding]
GO:0009887 [animal organ morphogenesis]
GO:0010506 [regulation of autophagy]
GO:0010628 [positive regulation of gene expression]
GO:0010742 [macrophage derived foam cell differentiation]
GO:0010821 [regulation of mitochondrion organization]
GO:0010976 [positive regulation of neuron projection development]
GO:0016032 [viral process]
GO:0016407 [acetyltransferase activity]
GO:0016573 [histone acetylation]
GO:0016579 [protein deubiquitination]
GO:0016740 [transferase activity]
GO:0016746 [transferase activity, transferring acyl groups]
GO:0018076 [N-terminal peptidyl-lysine acetylation]
GO:0018215 [protein phosphopantetheinylation]
GO:0018393 [internal peptidyl-lysine acetylation]
GO:0018394 [peptidyl-lysine acetylation]
GO:0030183 [B cell differentiation]
GO:0030220 [platelet formation]
GO:0030324 [lung development]
GO:0030511 [positive regulation of transforming growth factor beta receptor signaling pathway]
GO:0031333 [negative regulation of protein-containing complex assembly]
GO:0031490 [chromatin DNA binding]
GO:0031648 [protein destabilization]
GO:0032092 [positive regulation of protein binding]
GO:0032481 [positive regulation of type I interferon production]
GO:0032991 [protein-containing complex]
GO:0032993 [protein-DNA complex]
GO:0033613 [activating transcription factor binding]
GO:0034644 [cellular response to UV]
GO:0035257 [nuclear hormone receptor binding]
GO:0035264 [multicellular organism growth]
GO:0035855 [megakaryocyte development]
GO:0036268 [swimming]
GO:0042771 [intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator]
GO:0043627 [response to estrogen]
GO:0043923 [positive regulation by host of viral transcription]
GO:0043967 [histone H4 acetylation]
GO:0043969 [histone H2B acetylation]
GO:0045444 [fat cell differentiation]
GO:0045652 [regulation of megakaryocyte differentiation]
GO:0045721 [negative regulation of gluconeogenesis]
GO:0045747 [positive regulation of Notch signaling pathway]
GO:0045815 [positive regulation of gene expression, epigenetic]
GO:0045893 [positive regulation of transcription, DNA-templated]
GO:0045944 [positive regulation of transcription by RNA polymerase II]
GO:0046872 [metal ion binding]
GO:0048156 [tau protein binding]
GO:0048511 [rhythmic process]
GO:0050681 [androgen receptor binding]
GO:0050821 [protein stabilization]
GO:0051059 [NF-kappaB binding]
GO:0051091 [positive regulation of DNA-binding transcription factor activity]
GO:0051726 [regulation of cell cycle]
GO:0060325 [face morphogenesis]
GO:0060765 [regulation of androgen receptor signaling pathway]
GO:0061418 [regulation of transcription from RNA polymerase II promoter in response to hypoxia]
GO:0061733 [peptide-lysine-N-acetyltransferase activity]
GO:0061920 [protein propionyltransferase activity]
GO:0061921 [peptidyl-lysine propionylation]
GO:0090043 [regulation of tubulin deacetylation]
GO:0097157 [pre-mRNA intronic binding]
GO:0097677 [STAT family protein binding]
GO:0140065 [peptide butyryltransferase activity]
GO:0140066 [peptidyl-lysine crotonylation]
GO:0140067 [peptidyl-lysine butyrylation]
GO:0140068 [histone crotonyltransferase activity]
GO:0140069 [histone butyryltransferase activity]
GO:1900034 [regulation of cellular response to heat]
GO:1901224 [positive regulation of NIK/NF-kappaB signaling]
GO:1901796 [regulation of signal transduction by p53 class mediator]
GO:1904837 [beta-catenin-TCF complex assembly]
GO:1905636 [positive regulation of RNA polymerase II regulatory region sequence-specific DNA binding]
Show all
2414 aa
264.2 kDa
No 0
EP300-203
ENSP00000489397
ENST00000634690
A0A0U1RR87 [Direct mapping]
Histone acetyltransferase p300
Show all
Metabolic proteins
Predicted intracellular proteins
   Intracellular proteins predicted by MDM and MDSEC
Cancer-related genes
   Mutated cancer genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Other Mutations
   COSMIC Nonsense Mutations
   COSMIC Missense Mutations
   COSMIC Frameshift Mutations
   COSMIC Translocations
Human disease related genes
   Cancers
   Cancers of haematopoietic and lymphoid tissues
   Congenital malformations
   Other congenital malformations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003712 [transcription coregulator activity]
GO:0005634 [nucleus]
GO:0006355 [regulation of transcription, DNA-templated]
Show all
187 aa
20.5 kDa
No 0
EP300-212
ENSP00000501078
ENST00000674155
A0A669KB12 [Direct mapping]
Histone acetyltransferase
Show all
Metabolic proteins
Predicted intracellular proteins
   Intracellular proteins predicted by MDM and MDSEC
Cancer-related genes
   Mutated cancer genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Other Mutations
   COSMIC Nonsense Mutations
   COSMIC Missense Mutations
   COSMIC Frameshift Mutations
   COSMIC Translocations
Human disease related genes
   Cancers
   Cancers of haematopoietic and lymphoid tissues
   Congenital malformations
   Other congenital malformations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000123 [histone acetyltransferase complex]
GO:0003712 [transcription coregulator activity]
GO:0003713 [transcription coactivator activity]
GO:0004402 [histone acetyltransferase activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005737 [cytoplasm]
GO:0006355 [regulation of transcription, DNA-templated]
GO:0008270 [zinc ion binding]
GO:0016573 [histone acetylation]
GO:0016740 [transferase activity]
GO:0016746 [transferase activity, transferring acyl groups]
GO:0045893 [positive regulation of transcription, DNA-templated]
GO:0046872 [metal ion binding]
GO:0048511 [rhythmic process]
Show all
2388 aa
261.4 kDa
No 0

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The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg Foundation.