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PIK3CA
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  • PIK3CA
PROTEIN STRUCTURE
ANTIBODIES
AND
VALIDATION
Protein structures
GENERAL INFORMATIONi

General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.

Gene namei

Official gene symbol, which is typically a short form of the gene name, according to HGNC.

PIK3CA
Synonyms PI3K
Gene descriptioni

Full gene name according to HGNC.

Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
Protein classi

Assigned HPA protein class(es) for the encoded protein(s).

Read more
Cancer-related genes
Disease related genes
Enzymes
FDA approved drug targets
Human disease related genes
Metabolic proteins
RAS pathway related proteins
Predicted locationi

All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions.

  • Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached.

  • Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s).

The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location.

Read more
Intracellular
Protein evidence Evidence at protein level (all genes)
GENE INFORMATIONi

Gene information from Ensembl and Entrez, as well as links to available gene identifiers are displayed here. Information was retrieved from Ensembl if not indicated otherwise.

Chromosome 3
Cytoband q26.32
Chromosome location (bp) 179148114 - 179240093
Number of transcriptsi

Number of protein-coding transcripts from the gene as defined by Ensembl.

4
Ensembl ENSG00000121879 (version 103.38)
Entrez gene 5290
HGNC HGNC:8975
UniProt P42336 (UniProt - Evidence at protein level)
neXtProt NX_P42336
Antibodypedia PIK3CA antibodies


PROTEIN STRUCTUREi

The Structure section provides predicted structures from the Alphafold protein structure database and available experimental structures from Protein Data Bank (PDB).

In the Structure drop-down menu all experimental structures from PDB are available for selection and display. The structures are displayed using the NGL Viewer and can be zoomed-in and rotated either manually or by checking the Autorotate box. The Color scheme can be selected to show the residue index, chain name or confidence score (as B-factors and pLDDT score for experimental and predicted structures, respectively). The positions for available antigen sequences in the structure are shown if Antigens is turned to ON, and the Variants slider can be used to show the positions of clinical and population variants.https://github.com/nglviewer/ngl

Read more

Predicted


Description: Structure prediction from Alphafold project. Structure version 2

# Chains: 1      # Clinical variants: 45      # Population variants: 240

Experimental

Description: Discovery of a potent and isoform-selective targeted covalent inhibitor of the lipid kinase PI3Kalpha (X-ray)

# Chains: 1      # Clinical variants: 0      # Population variants: 0

Antigens:

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On

Variants:

Off
Clinical
Population

Color scheme:

Confidence
Residue index
Chain name

Autorotate:

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On



PROTEIN BROWSERi

The protein browser displays the antigen location on the target protein(s) and the features of the target protein. The tabs at the top of the protein view section can be used to switch between the different splice variants to which an antigen has been mapped.

At the top of the view, the position of the antigen (identified by the corresponding HPA identifier) is shown as a green bar. A yellow triangle on the bar indicates a <100% sequence identity to the protein target.

Below the antigens, the maximum percent sequence identity of the protein to all other proteins from other human genes is displayed, using a sliding window of 10 aa residues (HsID 10) or 50 aa residues (HsID 50). The region with the lowest possible identity is always selected for antigen design, with a maximum identity of 60% allowed for designing a single-target antigen (read more).

The curve in blue displays the predicted antigenicity i.e. the tendency for different regions of the protein to generate an immune response, with peak regions being predicted to be more antigenic.The curve shows average values based on a sliding window approach using an in-house propensity scale. (read more).

If a signal peptide is predicted by a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius (turquoise) and/or transmembrane regions (orange) are predicted by MDM, these are displayed.

Low complexity regions are shown in yellow and InterPro regions in green. Common (purple) and unique (grey) regions between different splice variants of the gene are also displayed (read more), and at the bottom of the protein view is the protein scale.
PIK3CA-201
PIK3CA-203
PIK3CA-204
PIK3CA-205


PROTEIN INFORMATIONi

The protein information section displays alternative protein-coding transcripts (splice variants) encoded by this gene according to the Ensembl database.

The ENSP identifier links to the Ensembl website protein summary, while the ENST identifier links to the Ensembl website transcript summary for the selected splice variant. The data in the UniProt column can be expanded to show links to all matching UniProt identifiers for this protein.

The protein classes assigned to this protein are shown if expanding the data in the protein class column. Parent protein classes are in bold font and subclasses are listed under the parent class.

The Gene Ontology terms assigned to this protein are listed if expanding the Gene ontology column. The length of the protein (amino acid residues according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius) and the number of predicted transmembrane region(s) (according to MDM) are also reported.
Splice variant UniProt Protein class Gene ontology Length & mass Signal peptide
(predicted)
Transmembrane regions
(predicted)
PIK3CA-201
ENSP00000263967
ENST00000263967
P42336 [Direct mapping]
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Show all
Enzymes
   ENZYME proteins
   Transferases
   Kinases
   Atypical kinases
Metabolic proteins
Predicted intracellular proteins
   Intracellular proteins predicted by MDM and MDSEC
RAS pathway related proteins
Cancer-related genes
   Candidate cancer biomarkers
   Mutated cancer genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Missense Mutations
Disease related genes
FDA approved drug targets
   Small molecule drugs
Human disease related genes
   Cancers
   Cancers of the digestive system
   Cancers of soft tissues and bone
   Cancers of the breast and female genital organs
   Congenital malformations
   Other congenital malformations
   Endocrine and metabolic diseases
   Hypothalamus and pituitary gland diseases
   Adrenal gland diseases
Mapped to neXtProt
   neXtProt - Evidence at protein level
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000166 [nucleotide binding]
GO:0001525 [angiogenesis]
GO:0001889 [liver development]
GO:0001932 [regulation of protein phosphorylation]
GO:0001944 [vasculature development]
GO:0004674 [protein serine/threonine kinase activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0005942 [phosphatidylinositol 3-kinase complex]
GO:0005943 [phosphatidylinositol 3-kinase complex, class IA]
GO:0005944 [phosphatidylinositol 3-kinase complex, class IB]
GO:0006006 [glucose metabolic process]
GO:0006468 [protein phosphorylation]
GO:0006661 [phosphatidylinositol biosynthetic process]
GO:0006909 [phagocytosis]
GO:0007173 [epidermal growth factor receptor signaling pathway]
GO:0007186 [G protein-coupled receptor signaling pathway]
GO:0007411 [axon guidance]
GO:0010468 [regulation of gene expression]
GO:0010592 [positive regulation of lamellipodium assembly]
GO:0014065 [phosphatidylinositol 3-kinase signaling]
GO:0014068 [positive regulation of phosphatidylinositol 3-kinase signaling]
GO:0014704 [intercalated disc]
GO:0016020 [membrane]
GO:0016242 [negative regulation of macroautophagy]
GO:0016301 [kinase activity]
GO:0016303 [1-phosphatidylinositol-3-kinase activity]
GO:0016310 [phosphorylation]
GO:0016477 [cell migration]
GO:0016740 [transferase activity]
GO:0019221 [cytokine-mediated signaling pathway]
GO:0030027 [lamellipodium]
GO:0030036 [actin cytoskeleton organization]
GO:0030168 [platelet activation]
GO:0030295 [protein kinase activator activity]
GO:0030835 [negative regulation of actin filament depolymerization]
GO:0031295 [T cell costimulation]
GO:0032008 [positive regulation of TOR signaling]
GO:0032147 [activation of protein kinase activity]
GO:0033138 [positive regulation of peptidyl-serine phosphorylation]
GO:0035004 [phosphatidylinositol 3-kinase activity]
GO:0035005 [1-phosphatidylinositol-4-phosphate 3-kinase activity]
GO:0035994 [response to muscle stretch]
GO:0036092 [phosphatidylinositol-3-phosphate biosynthetic process]
GO:0038028 [insulin receptor signaling pathway via phosphatidylinositol 3-kinase]
GO:0038084 [vascular endothelial growth factor signaling pathway]
GO:0038095 [Fc-epsilon receptor signaling pathway]
GO:0038096 [Fc-gamma receptor signaling pathway involved in phagocytosis]
GO:0038128 [ERBB2 signaling pathway]
GO:0040014 [regulation of multicellular organism growth]
GO:0043276 [anoikis]
GO:0043457 [regulation of cellular respiration]
GO:0043491 [protein kinase B signaling]
GO:0043524 [negative regulation of neuron apoptotic process]
GO:0043542 [endothelial cell migration]
GO:0043560 [insulin receptor substrate binding]
GO:0044029 [hypomethylation of CpG island]
GO:0046854 [phosphatidylinositol phosphorylation]
GO:0046934 [phosphatidylinositol-4,5-bisphosphate 3-kinase activity]
GO:0048010 [vascular endothelial growth factor receptor signaling pathway]
GO:0048015 [phosphatidylinositol-mediated signaling]
GO:0048471 [perinuclear region of cytoplasm]
GO:0050852 [T cell receptor signaling pathway]
GO:0050900 [leukocyte migration]
GO:0051897 [positive regulation of protein kinase B signaling]
GO:0052742 [phosphatidylinositol kinase activity]
GO:0052812 [phosphatidylinositol-3,4-bisphosphate 5-kinase activity]
GO:0055119 [relaxation of cardiac muscle]
GO:0060048 [cardiac muscle contraction]
GO:0060612 [adipose tissue development]
GO:0071333 [cellular response to glucose stimulus]
GO:0071464 [cellular response to hydrostatic pressure]
GO:0086003 [cardiac muscle cell contraction]
GO:0097009 [energy homeostasis]
GO:0106310 []
GO:0106311 []
GO:0110053 [regulation of actin filament organization]
GO:2000270 [negative regulation of fibroblast apoptotic process]
GO:2000653 [regulation of genetic imprinting]
GO:2000811 [negative regulation of anoikis]
Show all
1068 aa
124.3 kDa
No 0
PIK3CA-203
ENSP00000417479
ENST00000468036
C9JAM9 [Direct mapping]
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Show all
Metabolic proteins
Predicted intracellular proteins
   Intracellular proteins predicted by MDM and MDSEC
RAS pathway related proteins
Cancer-related genes
   Mutated cancer genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Missense Mutations
Human disease related genes
   Cancers
   Cancers of the digestive system
   Cancers of soft tissues and bone
   Cancers of the breast and female genital organs
   Congenital malformations
   Other congenital malformations
   Endocrine and metabolic diseases
   Hypothalamus and pituitary gland diseases
   Adrenal gland diseases
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0046854 [phosphatidylinositol phosphorylation]
GO:0048015 [phosphatidylinositol-mediated signaling]
Show all
118 aa
13.8 kDa
No 0
PIK3CA-204
ENSP00000418145
ENST00000477735
C9J951 [Direct mapping]
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Show all
Metabolic proteins
Predicted intracellular proteins
   Intracellular proteins predicted by MDM and MDSEC
RAS pathway related proteins
Cancer-related genes
   Mutated cancer genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Missense Mutations
Human disease related genes
   Cancers
   Cancers of the digestive system
   Cancers of soft tissues and bone
   Cancers of the breast and female genital organs
   Congenital malformations
   Other congenital malformations
   Endocrine and metabolic diseases
   Hypothalamus and pituitary gland diseases
   Adrenal gland diseases
Protein evidence (Ezkurdia et al 2014)
Show all
21 aa
2.4 kDa
No 0
PIK3CA-205
ENSP00000493507
ENST00000643187
A0A2R8Y2F6 [Direct mapping]
Phosphatidylinositol-4,5-bisphosphate 3-kinase
Show all
Metabolic proteins
Predicted intracellular proteins
   Intracellular proteins predicted by MDM and MDSEC
RAS pathway related proteins
Cancer-related genes
   Mutated cancer genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Missense Mutations
Human disease related genes
   Cancers
   Cancers of the digestive system
   Cancers of soft tissues and bone
   Cancers of the breast and female genital organs
   Congenital malformations
   Other congenital malformations
   Endocrine and metabolic diseases
   Hypothalamus and pituitary gland diseases
   Adrenal gland diseases
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0016301 [kinase activity]
GO:0016310 [phosphorylation]
GO:0016740 [transferase activity]
GO:0046854 [phosphatidylinositol phosphorylation]
GO:0046934 [phosphatidylinositol-4,5-bisphosphate 3-kinase activity]
GO:0048015 [phosphatidylinositol-mediated signaling]
GO:0052812 [phosphatidylinositol-3,4-bisphosphate 5-kinase activity]
Show all
1000 aa
116.1 kDa
No 0

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The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg Foundation.