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Diffuse synaptic staining in many but not all brain regions, with various intensities. The strongest immunoreactivity is found in the hippocampus, thalamus, BNST and the granular layer of the cerebellum. In the neocortex, moderate staining is seen in the layers I-III and V. Staining intensity is high in the anterior hypothalamus but very low in the tuberal hypothalamus. The basal ganglia are sparsely stained with the exception of the shell of the nucleus accumbens. In the brainstem, the staining is sparse or absent, with the excpetion of some distinct nuclei/regions, like the lateral reticular nucleus, cochlear nucleus, colliculus superior, ventral spinocerebellar tract and the periaqueductal gray matter. In the olfactory bulb, glomerular-, external plexiform- and the mitral cell layer is strongly immunoreactive, the internal plexiform layer exhibit lower intensity staining while the granular layer exhibit very low intensity staining.
Positive cells and structuresi
Manually selected location of the protein positivity, observed by immunofluorescence staining in mouse brain.